Scientific Brief: Lessons in Proteomics in Quality Assurance and Quality Control

Author: Sushmita Mimi Roy

This brief is the summary of a presentation given by Sushmita Mimi Roy at the Brooks Life Science Systems Corporate Symposium at ISBER 2015 on May 5, 2015.

Proteomics can contribute significant data about quality control and assurance when it comes to the viability of biological samples. Proteomics is defined as a branch of science where people study protein structure, function, and interactions, and how proteins are organized in cells, tissues, and organisms. For the sake of simplicity, this paper will focus on technologies that have been used previously to annotate and measure the variety and amounts of different proteins in complex matrices such as human blood specimens.

Proteomics is a good indicator of sample quality. It shows if some proteins are going out of solution, what proteins are precipitating, which ones are staying in solution, and how much change has occurred. It tells us if some proteins are increasing and the relative amount of increase, the change in disulphide bonds, and whether proteolysis or deamadation is occurring. These are all changes that mass spectrometry has been successfully utilized to quantify.

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